I want to know that can we use the cryotop device for vitrification in oocytes once we have thawed the already vitrified oocytes using this device...Is it recommended or not.....
I have reused them for research purposes only. I clean them in water and detergent followed by alcohol. I would not recommend to reuse them for patient specimens (clinical use).
Agree with both, we reuse them for research/training purposes only, with no problem. We wash with sterile MilliQ water followed by ethanol and then UV it for 30 min before use.