Marcia Ferraz

2 Questions 5 Answers 0 Followers

Questions related from Marcia Ferraz

Is there a way to reduce auto-fluorescence when fixing cells with Karnovsky fixative?

Hello, I need to fix my 3D cell culture (incubated with sperm cells), and after a pre-experiment I saw that the best fixative is the Karnovsky. But the auto-fluorescence rate is too high compared...

27 January 2015 7,393 4 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

17 January 2014 7,894 18 View