At firsy I want to make conditioned medium from Jurkat cell，than I can co-cultuer the medium and islet β cell.
But today I find that my islet β cell=INS-1 cell line，they come from Rat.
I‘m so sad，I konw I can't use two different species be co-cultured.
Even so，I still want to ask：Is there any possibilities that I can co-culture these cell lines?
Thanke U
and
forgive my Qinglish.
As no description about your purpose for coculturing, it would be inappropriate to make suggestion, but if just be evaluate the media component and simultaneous growth of two cell lines, I would say yes, these two can be cultured together in RPMI-1640, 10%FBS, with additional glutamine, sodium pyruvate and antibiotic of your choice.
Best.
Mu-Shen Chang
Thank U .
My purpose for coculturing is to assess the proliferative and apoptosis when islet β cells in an inflammatory environment. The inflammatory environment comes from conditioned medium of Jurkat cell.
I don't know if I can make them two cocultured.
Best wishes
Bei Zhong
I would say no problem at all, if any concerns, adapt Jurkat cell first in INS-1 cells complete growth medium (or vice-versa, however, I do recommend adapt Jurkat cells) to evaluate experiment condition and if secreted cytokines amount had changed upon treatment. Jurkat is not as attaching surface dependent as INS-1, therefore, if cell states and cytokines secreted had not change significantly after aforementioned process, then you're good to go.
Best.
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