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As title， I want some islet β cell line，MIN6 or NES2y are fine，preferably NES2y。 Any clue may help，Thank U
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I want to prepare conditioned medium from Jurkat cells. I will culture Jurkat cells in normal culture media, then activate it with PMA 50ng/ml+Iono 1ug/ml what should I do next？ Is there a protocol?
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As described above！ I extracted RNA from Jurkat cells and use RT-PCR to detect Tbet/GATA3/ROR-γt/FOXP3 expression. Even I did not stimulated the cells , the result of RT-PCR show that they can...
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