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Questions related from Bei Zhong
As title， I want some islet β cell line，MIN6 or NES2y are fine，preferably NES2y。 Any clue may help，Thank U
15 March 2024 2,869 0 View
At firsy I want to make conditioned medium from Jurkat cell，than I can co-cultuer the medium and islet β cell. But today I find that my islet β cell=INS-1 cell line，they come from Rat. I‘m so...
15 March 2024 1,955 3 View
I want to prepare conditioned medium from Jurkat cells. I will culture Jurkat cells in normal culture media, then activate it with PMA 50ng/ml+Iono 1ug/ml what should I do next？ Is there a protocol?
08 January 2024 7,776 1 View
As described above！ I extracted RNA from Jurkat cells and use RT-PCR to detect Tbet/GATA3/ROR-γt/FOXP3 expression. Even I did not stimulated the cells , the result of RT-PCR show that they can...
01 January 1970 3,318 0 View
