The comet assay is a great way of measuring overall DNA damage in individual cells. I have only worked in tissue culture of primary human skin cells, so I don't know how performing a comet assay would work if you have to do an immediate isolation of cells from tissues in order to run the assay. Will you collect cells to grow in culture and then perform the comet assay? Each cell type runs differently under the same conditions, so you would have to run small assays and see how the different cell types do. For example: melanocytes are less sensitive to longer runs (30m at 30V) compared to fibroblasts (20m at 30V). This is to prevent getting any tails in the control cells only.
Yes. You can do it with various tissue culture cells. We are doing with fish blood (not isolated cells). I have seen ref. using cells isolated by homogenized organs of mice and rat by checking the viability of cells. My concern is the validity of the results.
yes, you can determined the percentage of tail DNA (DNA Damage) of any organ or cell through comet assay. for more information you can read my paper entitled Genotoxicity and antioxidant enzyme activity induced by hexavalent
chromium in Cyprinus carpio after in vivo exposure, which have published in Drug and Chemical Toxicology