It is bit confusing.  After absorption thr intestine fatty acids are packed in chylomicrons in the form of triglycerides.  Body cells use the fatty acids from chylomicrons after the action of cell surface lipases.  This is my understanding.  Am i right to say all the cell types must be possessing these lipases?  

Lipoprotein lipase (LPL) is required to hydrolyze the acyl group from the glycerol backbone. Then, glycerol is measured colorimetrically..There are protocols for assaying TGs *triacylglycerols)...but they pre-treat with LPL and one cannot distinguish mono, di or triacylgycerols. SO, cells need LPL to utilize TGs. TG cannot be quantitated specifically..Oil red O stains TG droplets.

Cells directly use fatty acids (usually added in ester form to cell culture)

Attached pdf has details on TG assay. Hope this helps!

One point:

You may stain control cells with Oil Red O.

Then add the FA ester or the cholesterol-in DMSO. to cells (dose response).

Then, stain again with Oil Red O. 

If dye is extracted carefully--you can quantitate colorimetrically and find change in TG levels in control and treated cells. 

Hi Asavari, I think you can add lipids to cells. There are companies selling  lipid mixture  solution for cell cultures ( for serum-free culture). I don't know composition of these lipids.  You can have a look here 

https://www.peprotech.com/en-GB/Pages/Product/Lipid_Mixture_Solution/LM-200

regards,

Sanjay