Can carbonyl groups be removed from the cytosolic fraction?

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Where can i get videos of duchenne and non-duchenne smiles?

I'm looking for videos (or eventually photos) of duchenne and non-duchenne smiles that I can use in my research for my thesis about influence of social exclusion on recognition of real and fake...

16 July 2023 1,972 1 View

How to precipitate and purify magic-size ZnTe nanoparticles?

In synthesis with a hydrophobic solvent and octadecylamine as a surface ligand. When methanol is added, the suspension becomes turbid, perhaps the nanoparticles agglomerate.

27 March 2023 3,797 2 View

Types of vertical wind turbines ?

Is there any comprehensive review on types of vertical wind turbines?

20 February 2023 3,071 3 View

Whether multiple elutions can increase the amount of isolated DNA (QIAamp Circulating Nucleic Acid)?

Hello all, I have a question concerning the elution step during the isolation of cfDNA with QIAamp Circulating Nucleic Acid Kit. We care a lot about the high efficiency of isolation of cfDNA and...

16 February 2023 6,943 0 View

Fast green in the staining of formalin fixed trochophores?

I have read that fast green is used for mortality/survival assessment of zooplankton in environmental samples. Will this dye work with formalin fixed samples?

16 March 2022 3,356 0 View

Does the RWA Scale (Altemeyer, 1981) has a subscales (e.g authoritarian agression, authoritarian submission, conventionalism)?

Good morning, I have a question about Robert Altemeyer's RWA scale. As far as I know, Robert Altemeyer defines right wing authoritarianism as a combination of conventionalism, authoritarian...

10 February 2022 5,123 1 View

Is there any tool for adults to measure left-wing authoritarianism?

Good morning, I am a psychology student and I want to do a research about attitudes to eugenic abortion and authoritrianism. As far as I know there is RWA and RWAS to measure right-wing...

08 February 2022 1,515 2 View

Are you interested in working with me on an article about social media in business management?

I am looking for people interested in conducting research on social media with the possibility of publishing on Wos. I'm open for suggestions

27 May 2021 4,216 8 View

Can I analyse Likert scale with one question per factor?

I'm in a pickle regarding my research project analysis in SPSS. I have constructed a questionnaire, measuring 6 different motivators for shopping* and I want to know if there is a difference...

09 March 2021 4,975 5 View

How can I overcome wrongly filled orbitals in ROHF calculation in ORCA? CAS/NEVPT?

Hi all! I write with this problem I am facing for few weeks now. I am doing ROHF calculations for enzyme's active center that contains iron ion as cofactor. I use ORCA software. I try to get...

02 April 2020 7,090 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pubmed/10695989

https://www.frontiersin.org/articles/10.3389/fphar.2019.00600/full

Thanks

Justyna Świeżak

Isam Eldin Hussein Elgailani thank you very much for the links, they're very helpful!