I work with RNA on the Bio-Rad Experion chip gel electrophoresis. Is it possible to determine strand length from the concentration results? I have noticed that other papers using this or Agilent don't usually report bp. Is this over reach when reporting total RNA quantity and RIN?
see these are approxiamte estimations and all depended upon your refernce marker.. that u load, however for library preparations the intensity in electrophoregam after fragnmentation of sample is important and approximate estimations are more than enough for that
can u add an electrophogram and see
@Sandeep
The reference markers come with the kit, and the manual gives marker concentration. I will have to check again, but I don't believe the marker's fragment lengths are mentioned. Here is an older gel I ran. One is the approximate gel rendition, and the other is the absorbance of the marker lane. I also added a sample well electrophoretogram to my original question.
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