Can BHT be substituted with any other antioxidant in lipid extraction?

More Kanchan Samadhiya's questions See All

What are the genuine real-life applications of metaheuristic algorithms currently being used beyond academic research papers?

I am seeking information about the 'actual' real-life applications of metaheuristic algorithms, specifically beyond academic research papers. I understand that these algorithms are powerful...

07 June 2024 9,991 4 View

Need help for thesis on land use suitability analysis for future urban development ?

I am Kanchan Tiwari, student of master of urban planning from MITS Gwalior. My research topic is 'land suitability analysis' for future urban development. My case study area is counter magnet...

07 July 2023 1,651 2 View

Which Research Methodology is highly followed and accepted ?

Generally we use different methodology according to our subject requirement but some time we feel same methodology is used by 2 different authors and the finding are totally different.

21 June 2023 2,447 3 View

What is the best temperature and incubation time for successful ligation of blunt ends?

I have performed it at 16 degree for overnight and at 21degree for 2 hours but not getting appropriate results.

25 August 2022 4,547 4 View

Not getting band on gel after elution of digested product. Why?

Elution of two digestions A (1.5+5kb) and B (10kb) was performed at same time with same kit, getting band of good concentration for A (1.5 the required one) but not getting any results for B. We...

25 August 2022 3,075 1 View

Can we use acidic buffer for hydration of dry film ? What will be the effect of acidic environment in liposome and its encapsulation efficiency ?

Is it necessary to maintain neutral pH of buffers for hydration?

21 February 2022 3,328 1 View

Can anyone tell me how to calculate quantity of PHA (PHB or PHV) by GC-FID?

Hello, I am trying to quantify PHA in microbes using GC-FID. I am using PHB-PHV co-polymer and benzoic acid from sigma as standards. The concentration of internal standard (benzoic acid) is 500...

23 October 2021 8,009 2 View

Kd from Fluorescence titration method and SPR differs by 1000-fold?

I have calculated the kd value for peptide (13 mer peptide) and LPS (Lipopolysaccharide) interaction through SPR and fluorescence displacement assay and got 1000 fold difference. the equilibrium...

20 October 2021 8,359 3 View

May I get help with VASP calculations for structure optimisation of Fe(1 1 0) slab of 5 layer and 80 atoms?

I am getting different energy values even if I give converge file as input second time. Please suggest changes if required. INCAR file is attached. Your help will be highly appreciated.

29 August 2021 9,713 4 View

My agrobacterium (LBA4404 pCambia **) stabs are not growing on both LB and LA?

I did streak plate from glycerol stock. Cultures were grown on plate and in LB, they grew well initially. But when I reinoculated glycerol stock after 3 months on LB and LA media their is no sign...

24 August 2021 7,006 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How can I measure arsenic concentration in a patient blood?

I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.

23 July 2024 3,654 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Monjurul Haq

Hello Kanchon,

It would be better if you mention the extraction method. BHT and BHA both are good for resisting oil oxidation but excessive dose affects health. So, I suggest you to use ascorbic acid or vitamin E.

Kanchan Samadhiya

Thanks Monjurul Haq.

I will be using direct trasesterification or Floch method.

Maria Stacewicz-Sapuntzakis

Pyrogallol added before homogenization.

Rasool Kamal

Mention the extraction method? why you not use the chloroform and methanol method 2:1 and later you can get pure lipids after vacuum pressure evaporation of chloroform.

Rasool Kamal,

I am using insitu transesterification method Methanol/HCl method and direct transesterification method

Thanks