I did  biofilm formation  for  staphylococcus.pseudintermedius  using microtiter plate method , i observed the biofilm formed on the ring of well of plate and slightly on the base , did  this correct or no? as we no S.pseudintermedius like  s.aureus should formed biofilm on the base of wells right after staining by 0.1% crystal violet right, any one agree with you? and what is your explanation for this results?

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