Hello Maryam Ali Hussein,
The solvent system to be used depends on the polarity of your compound. For instance, if your compound has protected groups, you might test a series of solvent systems starting with a less polar one in different ratios (DCM/Hex, EtOc/Hex). Also, avoid a concentrated spot in the baseline before elution. What might happen is a mixture of two or more compounds with almost similar Rfs. In that case, before a chromatograph purification, test different ratios of solvent systems.
The streaking pattern looks odd. My first thought was maybe there is something wrong with the plates themselves. Indeed, there are other kinds of stationary phase for tlc plates. I just read something that says to use Cellulose or 'kieselguhr' for carbohydrates and sugars. I've never tried that, myself. Perhaps a neighbor has some different plates you can use?
First, solvent system is not suitable for separation. From the TLC, not only can the product not be separated, but there may also be solubility problems. Second, the TLC plate may also have problems.
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