I want to perfom in-vitro drug delivery for my specimen . tell me any simplest method to check drug release fromulation .. simple method or any in direct estimation method.. because I dont have digestor apparatus.. !!
There is no simple method as far as I am aware, and the value of in vitro experiments when you are seeking to understand bioavailability is limited. You also mention drug delivery and drug release in the same sentence. Bioavailability is a consequence of solubility and a host of other factors, so the best place for you to start would be to check its solubility. There are also several software packages which will calculate the solubility and even estimate the bioavailability. In this way you will not have to do any experiments. When I have previously been in this situation I have sent my sample to a drug lab for testing - these services are not expensive and can be the first step towards establishing the PK parameters of your compound.
If you mean the release of drug from a particular formulation, the standard method is dissolution apparatus following USP guidelines.
If you mean measuring drug bioavailability in vitro. The best method I know involves placing an appropriate tissue between two chambers, one containing the API, the other an appropriate buffer. The effusion of the drug across the membrane is measured as a function of time.
Of course, these are just approximations and the best information probably comes from an suitable animal model.
Computational models are nice, but they are only as good as the data they use to scrutinize a new molecular entity for start. The other factors are the particular algorithms used to estimate attributes. If you have a large library to screen, than I would recommend this approach. If you have a single entity, I would recommend either the in vitro tissue permeation or the dissolution.
Hope that helps!
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