I have a protein which is stable in Citrate buffer. I want to know the concentration of Citrate in human serum to to set up the reaction. Please tell me if anyone knows with reference . Thanks
Hi,
Generally the concentration of citrate is ~145 µM in human serum. This attached link of an article may help you.
http://www.ncbi.nlm.nih.gov/pubmed/18181808
Thanks.
Citrate occurs in human serum at a concentration of about 0.1 mmol dm-3 .
You can further refer to the link below to obtain certain interesting informations:-
https://books.google.co.in/books?id=-k9Aqu4tA0QC&pg=PA28&lpg=PA28&dq=concentration+of+Citrate+in+human+serum&source=bl&ots=-hfgta9zWI&sig=DkhxZ6y2In9EC7hzgIOFlfz00iY&hl=en&sa=X&ved=0CD0Q6AEwBGoVChMI1YPdu53lxwIVyayUCh3ETw_t#v=onepage&q=concentration%20of%20Citrate%20in%20human%20serum&f=false
Thanks a lot Amit and Sandeep sir...
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