Can I use cochranelibrary for my meta analysis ?

Can I use cochranelibrary for my meta analysis ? Right now i have aggregated papers for my study using europmc which have open access. But i do see in cochranelibrary there are many studies...

03 February 2021 9,157 3 View

Which coordinate (inertial/spacecraft) is best useful for finding the plasma wave signatures in Parker Solar Probe (PSP) data?

The Parker Solar Probe (PSP) data is available in two coordinate systems, i.e., the interplanetary inertial coordinate RTN (Radial-Tangential-Normal) and the Spacecraft coordinate. I want to find...

17 September 2020 8,500 2 View

Dear all, Can anybody help me out to isolate p21 plasmid by alkaline lysis method ?

I couldn't able to isolate the plasmid and ended up getting only RNA. After repeating it for 2-3 times also I couldn't able to isolate p21 plasmid. I am trying to sort out the reason for this. I...

25 February 2018 1,281 2 View

Can any one plz suggest what are the ways to stabilize a protein?

I am expressing a protein 71 kDa in E.coli .After Ni-NTA purification it resulting 4-5 bands in SDS gel. After purifying through FPLC further also it is showing the same in SDS gel. I am unable to...

31 May 2016 3,342 7 View

Has anyone used E.coli shuffle T7 strain for recombinant protein expression?

Can you tell me about the growth condition, specific media requirement and the antibiotic resistant needed?

23 January 2015 504 4 View

How to run Femlab in MATLAB ?

I need to design an arbitrary waveguide using MATLAB. Femlab would be the right tool for this work. Is there any extra software needed to run my fem lab or is it inside the MATLAB? In command,...

14 March 2013 3,213 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View