Krushna Chandra Hembram

5 Questions 6 Answers 0 Followers

Questions related from Krushna Chandra Hembram

How to analyze ESI-QUAD-TOF data generated as .wiff file ?

We did a mass spectrometry analysis of a protein sample and got the result file as .wiff files. Can anybody suggest any software to analyze this data. Is there any protocol of how to analyze the...

17 March 2021 9,363 0 View

Dear all, Can anybody explain the reason for Peak shift towards right in cell cycle analysis ? G1-S-G2/M all towards right compared to control?

I was trying to do cell cycle analysis using MCF-7 cell lines in BD-Flow cytromery instrument. The peak of all phages are shifting towards right of treated cells as compared to normal MCF cells....

29 September 2018 2,357 3 View

Dear all, Can anybody help me out to isolate p21 plasmid by alkaline lysis method ?

I couldn't able to isolate the plasmid and ended up getting only RNA. After repeating it for 2-3 times also I couldn't able to isolate p21 plasmid. I am trying to sort out the reason for this. I...

26 February 2018 1,364 2 View

Can any one plz suggest what are the ways to stabilize a protein?

I am expressing a protein 71 kDa in E.coli .After Ni-NTA purification it resulting 4-5 bands in SDS gel. After purifying through FPLC further also it is showing the same in SDS gel. I am unable to...

01 June 2016 3,438 7 View

Has anyone used E.coli shuffle T7 strain for recombinant protein expression?

Can you tell me about the growth condition, specific media requirement and the antibiotic resistant needed?

24 January 2015 572 4 View