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Questions related from Krushna Chandra Hembram
In my study utilizing anti-Flag antibody to detect a Flag-tagged protein in HepG2 cell line via Western blotting, I have observed multiple bands beyond the expected molecular weight. I seek...
30 April 2024 199 1 View
We did a mass spectrometry analysis of a protein sample and got the result file as .wiff files. Can anybody suggest any software to analyze this data. Is there any protocol of how to analyze the...
17 March 2021 9,370 0 View
I was trying to do cell cycle analysis using MCF-7 cell lines in BD-Flow cytromery instrument. The peak of all phages are shifting towards right of treated cells as compared to normal MCF cells....
29 September 2018 2,365 3 View
I couldn't able to isolate the plasmid and ended up getting only RNA. After repeating it for 2-3 times also I couldn't able to isolate p21 plasmid. I am trying to sort out the reason for this. I...
26 February 2018 1,379 2 View
I am expressing a protein 71 kDa in E.coli .After Ni-NTA purification it resulting 4-5 bands in SDS gel. After purifying through FPLC further also it is showing the same in SDS gel. I am unable to...
01 June 2016 3,453 7 View
I am working on a protein which have a his tag in its c-term end. I have expressed it in E.Coli Strain. After purification by by Ni-NTA column..when i run the gel I saw 3-4 bands below the desired...
30 December 2015 8,245 10 View
Can you tell me about the growth condition, specific media requirement and the antibiotic resistant needed?
24 January 2015 586 4 View
