Dear Dr Sherlyn Putri Wijaya,

I think the different collagen extraction methods from fish skin, reported in this article (in attached), may actually help you in your research.

Best wishes,

Sabri

Please look at the following below link which may help you in your analysis:

http://www.arpnjournals.org/jeas/research_papers/rp_2015/jeas_1115_2905.pdf

Thanks

Isam Eldin Hussein Elgailani

Thank you for your kind answer. I have read the paper yet they don't give any information on how to prepare the sample for TLC. It seemed they used the sample as-is. What I would love to know is whether using the sample as it is will still produce single amino acid spots or not, and if not, how to obtain it.

@Mohamed Sabri Bensaad

Thank you for your kind answer. Will read it.