NEVER thaw viral stocks in a 37 degree water bath, always just thaw on ice
RSV is too unstable to thaw that slowly it needs to be quickly (less than 30s) swished in a 37 degree bath with some ice still present and thaw the rest on ice
Thaw on ice or just hold the stock in your hand it doesn’t matter
To give some context these stocks are in 1mL aliquots stored in 25% sucrose in a -80 freezer. Please help me! I need some reassurance before I run my plaque assay tomorrow and don’t have enough cells to test all techniques lol