I'm trying to make GelMA hydrogel and need it to be tagged/ dyed to visualize it using a fluorescent microscope. I wanted to know what dye to use ?

20 September 2022 0 5K Report

I was wondering if I should add the dye before or after photopolymerization.

I was wondering if I could use Nile blue?

Badges
Science topic

More Priyanka Sreeram's questions See All

I have got 22-25 colonies on my after transforming the ligation in DH5aplha. Should I screen all the colonies?

I have screened 10 colonies out of 22. But neither get insert release nor the PCR amplification for the desired gene. What should I do, should I further screen the colonies?

02 December 2020 3,225 3 View

Which bacteria can grow on 10x PBS buffer?

I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...

04 November 2020 9,427 6 View

Which model can be used to predict on below data?

I want to predict valuation using verbal SAS but there exists a strong nonlinearity between the two variables which makes unfit to use linear regression. Please see the attached scatter plot image...

05 August 2020 1,602 3 View

Which is the best model to predict student marks based on entrance exam result?

Hi, I have a dataset that contains student data that are nested based on different schools where more that one student belongs to a particular school. student_id school_id non_verbal...

29 July 2020 9,825 18 View

One step RT-PCR enzyme optimisation, can anyone help?

Helping others is the way we help help ourselves! In this case literally!! I work at a biotech company called Genaxxon Bioscience GmbH in Germany. We have an amazing one step RT-PCR enzyme which...

13 July 2020 7,395 2 View

Do Acetonitrile type (gradient grade or non gradient grade) affects the HPLC results?

Trying to develop the HILIC-RID method for sugar analyte but getting very different results between two-run of the same sample. Tried 60 minute washing between two-run. The mobile phase is 75:25...

11 June 2020 8,385 3 View

Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

20 February 2020 2,469 6 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

02 December 2019 5,582 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View