Can anyone suggest which is the best method to determine MIC for non sporulating fungi?

More Simi Jacob's questions See All

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

How to simulate a metalens with backward propagating source using a Lumerical FDTD?

Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...

14 June 2024 4,333 0 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Protocol for Coating Plates with Collagen?

Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.

22 April 2024 6,835 1 View

How do I determine and what radical is this in this EPR graph (DMPO)?

So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!

06 April 2024 7,084 3 View

Is it required to send interview transcriptions for publishing qualitative research in a journal as part of data availability?

As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?

03 March 2024 3,653 3 View

Which Promotor Sites can I use for In Vitro Transcription?

Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors...

26 February 2024 1,852 1 View

How should one teach ETHICS to the new generation of college students in 2024?

More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...

02 January 2024 7,862 6 View

Buffering pH of DNA Binding Solutions for Silica Columns?

I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...

03 December 2023 2,197 0 View

How do we design a three level dimensions of organizational ambidexterity?

I am currently exploring on the concept of "Organizational Ambidexterity". I would love you give me an insight on the way to design its dimensions in a three level scale, where it will depict its...

27 October 2023 3,058 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS?

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...

22 July 2024 5,334 6 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,420 3 View

How to interpret MIC values in microplate reader?

Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...

01 June 2024 7,920 3 View

Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

What concentrations should be selected for hemolytic assay for antimicrobials?

I am performing hemolysis assay for antimicrobials but there's difficulty in selecting the concentrations of the antimicrobials. I have read the papers and MHC 90 was determined. Should I consider...

21 May 2024 8,912 3 View

How to test MIC MBC with 96 wells plate?

How to test MIC MBC of protein with 96 wells plate?

09 May 2024 2,338 0 View

Which long preservation method(s) can I use for Stenocarpella maydis also Diplodia maydis? So that I remains intact and does not loose traits?

I am Senior Research Technician and I have some isolates of Stenocarpella maydis also Diplodia maydis. I am currently try to develop SOP for long term preservation, so that it does not loose its...

06 May 2024 5,809 4 View

Can the extraction liquid be used directly in bioactivity studies without removing the solvent?

Normally, after extracting a plant with solvents such as ethanol, methanol, and water, the solvent is removed, and the extracts are pulverized. A stock solution is then prepared from the powdered...

01 May 2024 2,424 9 View