Follow research article from Z.A.Shah based on optimized transient MCAO model. Procedure itself is complicated you will need atleast 10 mice to practice on. Prepare filament atleast 10 in number before starting surgery or you can buy filament depending on weight and age of mice.

Follow research article from Z.A.Shah based on optimized transient MCAO model. Procedure itself is complicated you will need atleast 10 mice to practice on. Prepare filament atleast 10 in number before starting surgery or you can buy filament depending on weight and age of mice.

Hi, 

Jove is a peer reviewed video-journal and has some nice video's on this, both for tMCAO in mice and rats.

http://www.jove.com/video/2879/intraluminal-middle-cerebral-artery-occlusion-mcao-model-for-ischemic

This video is a nice instruction video to perform the surgery in mice and illustrates the two methods for inducing MCAO. 

Some videos on the site are open access but it's best if your institution has a subscription.

An open access video for the rat MCAO procedure for example is this one: 

http://www.jove.com/video/1978/focal-cerebral-ischemia-model-endovascular-suture-occlusion-middle

Kind regards

We are doing a rabbit model. I think I am the only one teaching it right now.

Give me a call if you are interested.

Have done a reversible occlusion model in my doctoral studies. May be it's useful for you.