Low mRNA expression versus High Protein expression?

I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with several proteins (down-regulation at mRNA level and up-regulation...

09 June 2020 6,780 11 View

How to calculate my research impact factor including first and second authorship?

If I have published x no of papers as a first author and y no of papers as the second author, how can I calculate my research impact factor not considering the citation? Thank you in advance

30 May 2020 5,086 17 View

How to fix the low pressure problem in Ion Chromatography?

I am using Thermo Scientific Dionex ICS-1100. Even after purging(with N2) the tubes connected with Eluent Reservoir and Eluent Valve; Eluent Valve to Pump Heads & changing the eluent I am...

01 October 2019 8,954 2 View

Did anyone work with SKOV3 cells as in vitro model of Polycystic Ovarian Syndrome (PCOS)?

If anyone has used SKOV3 (ovarian cystadenocarcinoma cell line) as an in vitro system for Polycystic Ovarian Syndrome (PCOS), please share the journal reference and/or protocol used to establish...

07 August 2019 8,451 1 View

AC or DC coupling: which one is best for Solar plus energy storage projects?

Energy storage system can be added to Solar plants through AC coupling or DC coupling. Which one is convenient and easy ?

18 March 2019 1,528 4 View

Do the performance in terms of life cycle, energy density vary with different shapes (cylindrical, pouch and prismat) of same lithium ion chemistry?

Generally, most of the research papers talk about the performances of a Li-ion half cell, coin cell or pouch cell. Do those performances are comparable with other shapes (cylindrical, prismatic)...

21 May 2018 4,172 5 View

How Can i measure concentration of a particular Compound in an metabolite using NMR? I am new to NMR Spectroscopy. Please give a detailed answer?

I need to measure Concentration of a compound in cell extract. How can i do it. thanking you

20 November 2017 6,221 3 View

How does polymer addition affect the conductivity of surfactant solutions ?

I am adding PHPA polymer to cationic and non-ionic surfactant solutions and found the conductivity values to decrease. I wish to know why does it behave as such?

11 September 2017 7,169 3 View

What are hybrid nanoparticles ?

Hybrid NPs are composites consisting of 2 NPs at molecular level. But, I have doubts regarding its molecular structure, formulae and morphology, etc.

16 May 2017 8,218 3 View

Kindly explain the effect of added hybrid nanoparticles on the viscosity of surfactant solution?Does it also influence viscoelastic properties if any?

Research related answer discussing the rheological properties of a solution containing both surfactant and hybrid NPs, and compare with that of surfactant solution.

16 May 2017 655 1 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View