I am working to find a bioactive compound from a bacterial source which can show antifungal activity. So I have established the bacterial culture in a synthetic medium. What we found that the enzyme/proteinous compound is mainly extracellular in nature. Now I want to go for isolation, extraction and purification of the compound from the liquid culture of bacteria at laboratory scale. So I am asking to share your knowledge about the sort of suitable methods which will be helpful for getting the desired compound.
Dear Swaraj
there is no a simple and Universal answer to your question, it depends from the properties of the bioactive compounds (eg it is a protein, a small molecule, which is mw? pi? ) and of the media from which it need to be recovered and purified.
ciao
Manuele
Manuele Martinelli
Sir,
Thank you for your wise suggestion. Our interest is limited to the proteinous bioactive compounds(as we believe that most enzymes are proteinous in nature). And next is the bioactive compounds are mainly extracellular in nature(We have checked that by performing experiments). And once we extract it and then only we can find the MW & PI of the protein along with the estimation of the proteinous compound. We have the bacterial liquid culture(maintained in a synthetic media similar to LB media with minor modification).
So I want to know the general isolation & extraction procedure for gram positive bacteria(our bacteria belongs to Bacillus sp. ).
Dear Swarj you can isolate the desired compound by salting out method or ion exchange chromatography.
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