What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

12 December 2020 2,584 8 View

Why the IT services companies (based in India) are mainly patenting only at the USPTO?

Please suggest some papers/reports on the changes in the policy with respect to patenting of computer software in India. Does it have any implications as to why the IT services companies (mostly...

12 November 2020 8,351 1 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Can i use surfactant C-TAB in extraction and detection of the aminaphthalene sulfonates by HPLC technique?

In the detection of Aminonaphthalene sulfonates the quaternary ammonium salts like Tetrabutyl ammonium bromide ,Triethyl ammonium acetates are used so the C-TAB being an quarternary ammonium...

21 September 2020 9,636 1 View

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

27 August 2020 994 4 View

What is the critical chloride concentration for concrete made of GGBS and for concrete made with Silica fume ? Is it 0.5% ?

Ccrit or Cth values for concrete made with different mineral admixtures

21 July 2020 7,301 2 View

Objective function for Pulp library in python?

How do we define objective functions while doing optimization using Pulp in Python. We have defined Prediction models separately. Next step is to bring objective functions from prediction models...

06 July 2020 9,256 3 View

How i can dry Metal Nano crystals ?

freeze-drying is recommended for nanocrystals drying but other than this technique direct heat is useful to Metal Nano crytals

17 June 2020 1,238 3 View

I need to predict the time when a physical test on a machine will fail. Which time series forecast/ machine learning method should I use?

There are 2 columns: time(in seconds), amplitude of wave. I need to predict when the test may fail and we can observe a crack on the machine based on the amplitude. I do not know the...

30 May 2020 7,594 13 View

IDT or Geneart, which is best for full lenght gene synthesis?

Did anyone got full lenght genes synthesized from both IDT and Geneart and which one will be the best in terms of pricing, post purchase support and delivery speed. Preferably in India but the...

14 May 2020 942 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View