I have two data sets, one chloroplast SSR and one nuclear SSR, both with 20 populations and I would like to run a Barrier analysis (Barrier 2.2) separately once on nuclear and once on chloroplast. as I know I need a Fst or Gst matrix and a bootstrap matrix and GPS coordinates of the pops. Unfortunately, I don't know R software, so I need a something which can create these matrices from my data set. Any suggestions?

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