Does anyone know how to eliminate or reduce the interferences of nanoparticles with flow cytometric analysis of phosphorylated histone H2AX?

I am optimizing the analysis of the phosphorylated H2AX histone in cells exposed to nanoparticles using flow cytometry analysis and I observed some interferences of the nanoparticles with the...

27 March 2018 5,134 2 View

Why do my NIH 3T3 cells die after single cell sorting?

I have been sorting NIH 3T3 based on GFP positivity for quite a long time and this has worked fine when all the GFP positive cells are sorted to the same well (of a 24 well or 12 well plate). The...

01 June 2014 6,314 9 View

How fast should an ELISA test be?

18 January 2013 1,471 8 View

Can I re-incubate ELISA plates with primary Ab?

I'm running some ELISA experiment plates coated with an extremely expensive protein. However, at the end of the experiment there was no signal. The last step of this experiment was the incubation...

03 January 2013 5,979 5 View

Its possible to use the sample after western blotting?

11 April 2012 7,092 9 View

No title

How can I procede to separate glycprotein from serum?

21 September 2011 7,214 2 View

Someone knows the physiological causes of trend of this Lasiodiplodia isolate to the pink?

I replicate him and observed this trend. I want to know the physiological causes. In this picture can be hard to see it. Best regards,

01 January 1970 6,715 23 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Habitat Suitability through GIS without presence-absence data?

What kind of models/techniques can be used with GIS spatial analysis in order to determine habitat suitability for a species that is not (widely) present in a certain region? For my bachelor...

31 January 2021 2,777 2 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

Is there any procedure or an equation for converting total carbon to organic carbon or any equation that helps to obtain the value?

I have analysed percentage carbon (total) using CHNS Elemental Analyzer for the forest soils. Now, I would like to obtain SOC content from the obtained value for which I am looking for a procedure...

29 January 2021 5,551 8 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View