I am using LTR, MDC for the measurement of autophagy like processes. Though these dyes serve a lot but I want some dyes, if available, that sufficiently stain pre-autophagosomes, autophagosomes, and autolysosomes but not lysosomes. Thanks.
Sajjeev Jagannathan
http://www.enzolifesciences.com/ENZ-51031/cyto-id-autophagy-detection-kit/
These stain the pre-autophagosomes, autophagosomes, and autolysosomes but even though doesn't completely eliminate lysosomes, they stain them minimally.
The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes)
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