I would like to work on the beneficial role of angiogenesis in hypoxia induced neurodegeneration. I am very much confused in selection an important pathway or factor. Any suggestions regarding this will be very useful for me
Thank U in advance
Hif1a (hypoxia-inducible factor) is one of the more well-known transcription factors studied in the brain hypoxia field. Another downstream target of Hif1a, Vegf, is extremely well-studied, but I think reading the Vegf literature would not simplify your search in narrowing down an important pathway to study...
Look up the literature on how cancer cells regulate angiogenesis. Same factors.
You can look at VEGF pathway for sure. You can assay VEGF level, expression of VEGF receptors, particularly VEGFR2. Expression of HIF proteins. In addition, I would also look, for example, at the expression of CD105 (endoglin) if you are interested in studying the formation of new vessels. Would be good to know what type of experiments you want to set up in order to adapt the shot
My plan is to work on Angiogenesis induced neuroprotection following the cerebral ischemia in mice. I'll do functional assessments, histology, immunostaining (angiogenic markers). For this i need to choose some important molecules plays vital role in angiogenesis followed by ischemia.
I just wonder how you will do that since neuroprotection means that neurons are more resistant to cell death from ischemia and it is this ischemia that then upregulates angiogenesis afterwards (meaning days later) Most of the neurons should be dead by then since neurons rely on oxidative metabolism. A second problem is reperfusion that is initially devastating as well.
Good luck.
Look for Sirt1 as a potential angiogenic factor. Another pathway would be VEGF-eNOS-NO.
HIF-1 is certainly the most well known inducer of VEGF but i would suggest you to look for HIF-2 also, HIF-2 also regulates VEGF.
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