I think it will nice if you can use LC -QTOF MS/MS to identify your metabolites. Using QTOF MS you can determine the accurate mass of your metabolites, than using Isotope pattern the Molecular formula can be predicted, after searching in (online) data base (Metlin etc), and comparing their fragmentation,  the metabolite could be predicted. The metabolites can be further confirmed using authentic standards or (1D/2D-NMR after isolation)

Thank you Gunawan Indrayanto  sir,

i will try for this and will inform you the further work result.

sir,LC -QTOF MS/MS technology is not available in our class lab,,so have you any idea regarding the same topics?

Hi, if you do not mind, let me know what are the analytical equipment's which you have at your lab, With best wishes

Hplc, uv-vis spectrophotometer, AAS, FTIR spectrometer