Can anyone suggest me that what should be the conc. of HEPES buffer used for enzymatic studies?

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Mahesh Chandak

Dont worry, hepes show low absorption in uv-vis. You may try 20mM

Sumit Kumar

0.02M/0.2M Solution may be used. make a stock solution of 1.2 mM and dilute to 12 microM / 10 micro molar working solution. It will definitely work out.

If not working, then make 10 mM stock solution of your enzyme to be used and dilute to the corresponding 20/12/10 micro molar working solution.

Manpreet Kaur

thanks Mahesh chandak, Sumit sir and Sebastian Schmitt your answers are helpful to me..