Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

How to run Autodock 4 , using CONDA environment on server ?

#autodock #virtualscreening Question1: Am I doing this right , means do setting up conda on server works for virtual screening (AUTODOCK)? Question2: How can I modify the script (submit4.py)...

06 December 2020 2,424 3 View

Is it possible to isolate a His-tagged protein which has high prevalence of non-polar residues?

I am trying to express and isolate a protein in E. coli. The protein is supposed to have high prevalence of non-polar residues. Would that create problems in isolating the protein using a His tag...

08 September 2020 9,584 3 View

Can an isolated protein domain give a single band in SDS-PAGE equivalent to its dimeric mol. wt. ?

The protein I'm working with forms a homodimer and is a 2-domain protein. I have cloned and purified one of its domains. However, when I run the domain on SDS-PAGE, I get a single band equivalent...

19 August 2020 7,893 14 View

Can I prepare/download the dimeric structure of a protein which has a PDB file of the monomeric structure?

I want the dimeric structure of a protein (forms dimer) in .pdb format. The crystal structure of the protein is solved. However, the .pdb file at RCSB PDB is of the monomeric structure. Thanks...

09 July 2020 3,086 4 View

How to set the axis of fft2 in matlab for spatial wave?

I have some data of radiances over latitude and longitude. When we do fft, the frequency window is from -fs/2 to fs/2. But, when we do fft2, what will be in the x axis and y axis? -spatial...

16 November 2019 8,956 3 View

In DSP (digital signal processing) how can I retrive bits (data) after i have demodulated a signal to check BER (Bit Error Rate)?

Lets say, I have 10 randomly generated bits (0's and 1's) in matlab. From my other parameter calculations, I have about 5 samples, this means 1 bit/5 samples (each bit is replicated 5 times). My...

04 November 2019 6,514 3 View

What would be the best parameter to study a rising signal (say Calcium signals in live cells) ?

I am stimulating my cultured cells and recording calcium activity as fluorescence change using live-cell calcium imaging. Though I am more concerned about the decay of the signals, for which I am...

23 October 2019 3,842 6 View

Can anyone explain me why there is change in the Silver Nanoparticles UV-Vis max spectra before and after centrifuge ?

The final solution of AgNPs is pale yellow (I took the UV-Vis Spectra max) and then the solution was centrifuged thrice to obtain pure NPs i.e., dark colored (also took the UV-Vis Spectra max). So...

30 June 2019 7,758 5 View

Can someone share the protocol for calculating the extinction coefficient concentration of Silver Nanoparticles ?

I have synthesized AgNPs by citrate reduction method. The UV-vis max Peak is at 420 nm. Could anyone share the detailed protocol to calculate the concentration of the AgNPs ? Extinction...

01 June 2019 7,303 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View