How to fix error link 9999 in gaussian ? Its coming as I add a solvent IEFPCM/DCE Model to the job,without the solvent optimization is sucessful.Why?

I optimized the structure of Pd Acetate Trimer using HF/STO-3G and the B3LYP/LANL2DZ without any solvent. The optimization was successful without any negative frequency. Then I added solvent...

28 September 2020 745 3 View

Does Covid 19 exacerbate anaemia and diabetes in people ?

Can CRISPR-cas9 gene editing technology save patients suffering from low pancreatic beta cell and cure diabetes? If so any human trial going on?

26 June 2020 1,246 4 View

How do i separate a metal complex from DMSO?

Actually ,i couldnot evaporate DMSO due to high boiling point. So how can i get back my metal complex

01 March 2020 3,212 7 View

How to arrange the data for preparing Sample rarefaction (Mao tau) curve using PAST?

I have tree abundance data of six different land use categories and for each category i have taken 12 plots. Now I need to draw Sample rarefaction (Mao tau) curves showing all the six different...

19 September 2019 2,857 2 View

How to increase the adsorption of graphene oxide on any substrates?

I have deposited thin film of graphene oxide on corning glass and undoped Silicon substrates by dropcast method. I have kept at 50C for 3 hours to dry. Then I have annealed them at high...

03 October 2018 8,794 3 View

Is it possible to get smaller lattice parametrs in thin film form than bulk sample?

I deposited the thin films of MgTiO3 using RF sputtering onto quartz substrate. After XRD analysis of thin films and sputtering target (home made), it is observed that the lattice parameters of...

24 June 2018 4,327 2 View

What theory should be relevant to study the role of New Media in Democracy?

I have been working on a paper on the role of new media (specially social media) in democracy. My emphasis is on the role of social media in Indian Democracy. But could not able to use a...

16 June 2018 1,848 6 View

How to calculate the percentage crystallinity from XRD plot using origin software ?

I have calculated percentage crystallinity using ( Ac/Ac+Am ) formula for the following curves. But I am getting crystallinity percentage more for the sample C in comparison to A. Am I wrong in my...

21 September 2017 5,629 11 View

What are the substitutes to Sodium azide and EMS as cytotoxic agents?

What are the substitutes to Sodium azide and EMS as cytotoxic agents?

01 July 2017 6,909 1 View

I want to combine sequence of its and rbcl can anyone suggest me how to combine the sequences

i have ITS and Rbcl sequences and i want to analyse for combination of markers. can anyone suggest me how to do

20 May 2017 4,665 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View