Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

What are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems?

anyone suggest what are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems

21 January 2021 5,135 5 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

Was there any pesticide disasters other than Bhopal and Kasaragood tragedy around the globe?

Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...

11 January 2021 3,790 7 View

Suggest best performance evaluation metrics for image segmentation?

Good morning everyone. As a part of my research work, I designed a network that extracts the leaf region from the real field images. when I search about performance evaluation metrics for the...

10 January 2021 9,581 30 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

[Covid-19] GGO Evaluation - CT imaging of the lungs ?

13 January 2021 3,115 4 View

Can I measure lung weight after formalin fixation?

Hi, I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable? Thanks.

11 January 2021 3,178 3 View

Immortalized pulmonary microvascular endothelial cell to buy?

Hello, I would like to purchase an immortalized cell line for pulmonary microvascular endothelial cells (MVECs), but I could not find any. I looked up websites, like ATCC, Lonza, Cedarlane, and...

15 November 2020 8,085 3 View

Should I perform transcardial perfusion by PBS before lung isolation for RNA expression analysis?

Hey, I am planning to dissect rats and isolate Lung tissue samples to be stored in RNA stabilizer for later analysis. - Should I perform trandcardial perfusion by PBS ? - Is it necessary to...

21 September 2020 1,822 5 View

What are the correct boundary conditions for lungs in a breathing machine simulation?

Dear Colleagues, We are trying to simulate flow in a breathing machine and need to put the boundary conditions for lungs. Question: is it possible to set adequate boundary conditions for lungs,...

07 September 2020 8,670 4 View

What is the best software (for biomedical engineers) to design professional illustrations?

I am not a graphic designer! But now, I am looking for a Windows-based software to help me in the easy drawing of sophisticated 3D illustrations in the field of biomedical engineering and...

10 August 2020 5,034 7 View

How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

09 August 2020 4,967 1 View

CT Lung Image database for infected patients with COVID-19?

Dear Researchers, Could you please suggest me an available CT Lung Image database for infected patients with COVID-19. Thank you all in advance.

13 July 2020 9,733 6 View

Can solutions containing nanoparticles form an aeresol due to magnetic stirring or simple pouring?

Are nanoparticles in a liquid media generally safe for lungs and health?

17 May 2020 3,897 3 View