Dear

I recommend you protocol used by Itso 2011 entitled: Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge. ISME Journal (2011) 5, 1844–1856; doi:10.1038/ismej.2011.59.

Check also: Zehnder AJ, Huser BA, Brock TD, Wuhrmann K. (1980). Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol 124: 1–11.

Thank

http://www.nature.com/ismej/journal/v5/n12/full/ismej201159a.html

http://www.sciencedirect.com/science/article/pii/S1517838215000325

http://link.springer.com/article/10.1007%2FBF00407022

Thank you so much for your prompt response Mr.Marius K. Somda.