Mukesh Sankar

4 Questions 11 Answers 0 Followers

Questions related from Mukesh Sankar

How to isolate one plasmid from E. coli Bl21(DE3) containing two plasmids?

I have an E. coli BL21(DE3) strain which contains two plasmids (pCDF and pACYC). I need to isolate pACYC alone from this base strain. Could somebody suggest me an easy method? FYI, I already try...

05 May 2017 4,042 9 View

Can anyone suggest me a protocol to isolate an aerobic acetate utilizing bacteria from sludge?

I am working on isolating an aerobic acetate utilizing strain from sludge? Is there any marker to screen only acetate utilizing strain?

01 January 2017 5,332 2 View

Growth characteristics of E. coli RR1: can it be grown on minimal media containing glycerol as a carbon source?

I am studying the growth and acid tolerance of various E. coli strains. I am growing cells in minimal media containing glycerol as a carbon source. During this study, I have noticed that E. coli...

04 April 2013 8,095 5 View

I have over expressed a small protein (21 amino acids) in plasmid. Are there any methods to check its expression? The size of the protein is 2.3 Kda.

When i try to check its expression on 18% SDS gel, i could not find any band. Could anybody suggest a method ?

01 January 2013 1,455 8 View