Methyl green is a commonly used stain in histology, and while it is generally quite stable and binds strongly to DNA and proteins, it can be removed with the appropriate chemicals. Here is a general protocol for destaining methyl green from a slide:

Materials Needed:

• Xylene or a xylene substitute (e.g., Clear-X, cedarwood oil)
• 100% Ethanol
• 95% Ethanol
• Distilled water
• Coplin jar or a container large enough to hold the slides
• Slide warmer or water bath (optional)

Protocol:

Safety Precautions:

• Work in a well-ventilated area or under a fume hood when using xylene.
• Wear appropriate personal protective equipment (PPE), including gloves and safety goggles.
• Dispose of xylene and ethanol properly according to your institution’s safety guidelines.

Remember that some tissues may be more delicate than others, and over-destaining can lead to tissue damage. Always monitor the process and be prepared to stop if the tissue starts to show signs of damage.

Thank you for your suggestion Zhi Cao :)

I would rinse the slides in acidified water to remove the stain. It is also possible, that the methyl green will be solved in the hematoxylin-solution because of the low ph. just try.