I have already a polyethylene lid which is not efficient. I tried to use parafilm but the solvent still evaporates. I tried to put Aluminum foil then the lid on to make it a quick fit, but the solvent still evaporates.
There are cuvettes with tight seals. Perhaps you should get one of those. See for example Hellma 117.204F
http://goo.gl/962bSX
besides cells with 'tight seals' a further option (best in addition) maybe to determine concentration before and after the fluorescence measurement.
Add a bit of mineral oil e.g. from Sigma on top of your sample in the cuvette.
The teflon stopper is a best solution with the conventional cells on the market. However, take care about the change of the volume as result of the temperature change. In a large temperature range it can influence the concentration substantially.
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