Can anyone suggest how to establish a pre diabetic condition in vitro? I am particularly interested in skeletal muscle lines.

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John W. Wood

If you use C2C12 myoblasts, and allow them to differentiate/fuse into myotubes, you could theoretically use DMEM with different amounts of glucose for a set time and assay IRS phosphyorylation/ glucose uptake?

Dipsikha Biswas

I was looking for a concentration of glucose and fatty acids that could be used for an in vitro pre diabetes state. I do not want to make it glucolipotoxic to mimic the diabetic conditions, I was looking for an earlier stage than that.

Zsuzsanna Mezei

What sort of prediabetic conditions are you interested in? IFG or IGT? At this stage it's almost impossible to give an expected lipid status, better read about it and choose something you're interested in.