Can anyone suggest how much antibiotic dosage should be added to grow dental stem cells from an inflamed source in order to prevent contamination?

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Kay Samuel

when using infected tonsils as a source of lymphocytes we used to prepare a single cell suspension, wash once in 10x Pen and strep, wash twice in PBS and then culture cells in 1x Pen and strep. Usually this was sufficient to prevent contamination of cultured cells.

kay

Priyadarshni Bindal

Thanks Kay Samuel