Hello everybody, I am working in a conifer tree species, initially i need to propagate the plant through callus, i have established the callus induction but i struggle to regenerate the plant through callus, so i have planned to regenerate the plant through epigenetic remodeling. can we regenerate the plant through DNA methylation process..? i looked at the protocol for this. i would appreciate if anyone have experience in the plant regeneration via epigenetics. Thank you.
You may find useful these references:
Article Somatic embryogenesis — Stress-induced remodeling of plant cell fate
Article Dynamic Epigenetic Changes during Plant Regeneration
However, epigenetic remodelling is not something you can manipulate at will, it depends on the cell developmental state, the tissue type, the environmental conditions, and the hormonal stimuli present.
My advice is look for available regeneration protocols for close relatives of the species you want to regenerate and test how these work on your case.
Most trees, including conifers, have extremely large genomes with high duplication and tend to have a large number of transposable elements of various classes. It may be hard to promote plant embryogenesis from callus, and sometimes direct organogenesis is more effective.
An example:
https://www.sciencedirect.com/science/article/pii/S0304423817305605
I really do not get the point how and why you think that your hypothetical method would work. (epigenetic modelling for tissue culture)
Absolutely agree with Rogelio Rodríguez-Sotres 's answer above. Tissue culture needs very keen and wide screening for all the parameters mentioned above, and get the right configuration. Rest is mentioned above.
@ Rogelio Rodríguez-Sotres , Thank you very much for your
kind response sir.
@ Abhijeet Singh , Thank you for your suggestion sir.
- Badges
- Science topic
- Similar topics
- Biological Science
- Histology
- Sectioning