You should try the BioVinci application. Just prepare a matrix of samples as columns, cytokine data as rows in tsv or csv format. Then import the file to BioVinci and use the Hierarchical clustering. Click here to try the heatmap example in BioVinci now: https://vinci.bioturing.com/panel/workset/build/hierarchical-clustering

One option is Plotly (https://plot.ly/). It requires creating an account but it is free.

There's a guide to creating heatmaps with Plotly here: https://plot.ly/how-to-make-a-heatmap-online/

depending on your data, you can scale the data to unit sd and then do heatmap in R.

jColorGrid (http://jcolorgrid.sourceforge.net/) is good for simple heatmaps.

Article JColorGrid: Software for the visualization of biological measurements

I'm sure the previous responders have the formal solutions covered, so attend to their comments first.  If you have time to ponder novelty, consider topological/threshold contour mapping in 2D.  (Wash/Rinse/Repeat (like OSEM in brain image reconstruction))  --- colorgrids--- good suggestion dww

you can isntall R pacakge, gplots which have heatmap.2 . it is easy to do with heatmap.2 function. please follow this http://mannheimiagoesprogramming.blogspot.in/2012/06/drawing-heatmaps-in-r-with-heatmap2.html

hope this will work for you.

http://www.tm4.org/mev.html

It's an easy to use standalone java program that generates excellent heatmaps.

R is great, but not for novices. If this is your first time making a heatmap, try MEV as Michael suggested, or Java TreeView (http://jtreeview.sourceforge.net/)

Also Try BRBtools which is available as excel plugin

Hello,

I use GENE_E and I find it very well 

http://www.broadinstitute.org/cancer/software/GENE-E/

GenEx ver 6 also a very good software for heatmap pls try it

You should try the BioVinci application. Just prepare a matrix of samples as columns, cytokine data as rows in tsv or csv format. Then import the file to BioVinci and use the Hierarchical clustering. Click here to try the heatmap example in BioVinci now: https://vinci.bioturing.com/panel/workset/build/hierarchical-clustering

heat mapper (http://www.heatmapper.ca/) may be is another option

I used Gitools http://www.gitools.org/ to represent heatmap data

Alessandro

I use this one https://www.hiv.lanl.gov/content/sequence/HEATMAP/heatmap.html

You can use MeV 4.9 software for construction of heatmaps. It is easy to use.

ClustVis: https://biit.cs.ut.ee/clustvis/