Can anyone suggest an appropriate protocol for Vitamins assay from natural product along with its isolation and purification ?

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Guilherme Vannucchi Portari

Dear Arvind Singh Chandel,

mostly vitamins are identified and quantified by HPLC. As they are not of the same chemical group is difficult to apply one methodology that can extract and quantify it.

You can try to look these paper. I hope they are useful.

Guilherme

http://www.us.edu.pl/uniwersytet/jednostki/wydzialy/chemia/acta/ac15/zrodla/20_AC15.pdf

http://www.hindawi.com/journals/jchem/2014/831357/