Hi,

I am growing the SK-MEL-3 cells in DMEM-Hi Glucose with, 10% FBS, 2 mM Glutamine (this is additional to the 2 mM L-gln that is already supplied in the media), Pen-Strep, Gentamicin. But the cells are growing slowly.

I saw that McCoy's 5a Medium with 15% FBS is suggested by ATCC, but before switching to a new media, I was looking for alternative use of DMEM as most of the other cells are adapted to it.

Thanks in advance for the suggestions,

Debanjan