I need to reproduce symptoms of soilborne diseases (specially Fusarium rot) in marjoram plants, in order to get Koch's postulates done. However the inoculation of conidial suspensions or even pda colonies did not reproduce the symptoms. Can anybody suggest a suitable way to have success? Thanks!
i thank using thermal image is very good method for detection of fusarium disease because the physiology change which happen where infected with it
Ahmed, that's interesting for disease diagnostic, but my question is how to reproduce the symptoms... thanks!
Dear Gei,
If foliar inoculation is not working to produce symptoms after inoculation you can go for soil infestation method. As you stated, it is a soil borne, let us keep the inoculum in soil and grow your plants. If the pathogen is causing disease on aerial parts like stem (you can go for needle/tooth pick inoculation method). If it is associated with root rot then soil infestation will be the best option. As you used the term rot,i understands it is not associated with leaves though it will be affected by this pathogen. If all the possibilities fails, means we have to look for an alternative.
if you have any doubts feel free to share
good luck
As already told, you can inoculate the soil (firstly by conidial suspension) and produce some damage (with a different extent) to the roots, helping penetration of the pathogen.
Good luck!
Gianfranco Romanazzi
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