I have to use it for extracting chloroplast proteins.
Article An efficient method for the extraction of chloroplast protei...
You can use a mixture of chloroform/methanol in different ratio ,starting with less polar mixture.
Thank you.
I am trying to analyze my data by performing anova followed by tukey's hsd in excel but don't know how to get the significance letters.
03 April 2019 2,007 10 View
I am using the following protocol for measuring rubisco content: Large subunits and relevant small subunits were cut and washed with 3 mL of formamide in a 10 mL cuvette, then incubated in a 50...
03 April 2019 6,242 6 View
If a study uses 2 treatments for example 3 different types of biochars i.e. a, b and c are used to reduce the uptake of cadmium and lead by a crop. 2 doses are selected for each of the heavymetal...
02 March 2019 2,357 19 View
What affects the band intensity in sds gel? Does glycine concentration play a role? Or the pH of the running buffer? Or sample buffer volume and concentration?
01 February 2019 2,137 5 View
Sometimes when i run my samples, the dye becomes more diffuse and spreads out more when it enters the resolving gel, while other times it is much narrower and darker...Does it indicate anything,...
01 February 2019 8,570 5 View
While pouring both the resolving and the stacking gels inside the glass plates, bubbles are produced because of the sds due to which the wells do not form properly and so I can't load my samples...
09 October 2018 1,054 12 View
I have to use it for SDS-PAGE.
03 April 2018 7,159 10 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...
28 February 2021 7,330 4 View
28 February 2021 8,466 2 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...
28 February 2021 912 3 View