Can anyone suggest a substitute for dimethyl ether?

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Does BSA has any absorbance at 595 nm in the absence of bradford reagent?

I am using the following protocol for measuring rubisco content: Large subunits and relevant small subunits were cut and washed with 3 mL of formamide in a 10 mL cuvette, then incubated in a 50...

03 April 2019 6,294 6 View

How to get letters indicating significant difference using ANOVA in excel or spss?

I am trying to analyze my data by performing anova followed by tukey's hsd in excel but don't know how to get the significance letters.

03 April 2019 2,066 10 View

Need guidance regarding statistical analysis?

If a study uses 2 treatments for example 3 different types of biochars i.e. a, b and c are used to reduce the uptake of cadmium and lead by a crop. 2 doses are selected for each of the heavymetal...

02 March 2019 2,418 19 View

What else does the dye front indicate in SDS PAGE, except the migration of proteins?

Sometimes when i run my samples, the dye becomes more diffuse and spreads out more when it enters the resolving gel, while other times it is much narrower and darker...Does it indicate anything,...

01 February 2019 8,614 5 View

Does the concentration of SDS running buffer affect band intensity?

What affects the band intensity in sds gel? Does glycine concentration play a role? Or the pH of the running buffer? Or sample buffer volume and concentration?

01 February 2019 2,187 5 View

How to prevent bubble formation in the gels while performing SDS PAGE?

While pouring both the resolving and the stacking gels inside the glass plates, bubbles are produced because of the sds due to which the wells do not form properly and so I can't load my samples...

09 October 2018 1,108 12 View

Whats the easiest, feasible and most reliable protocol for protein extraction from plant leaves?

I have to use it for SDS-PAGE.

03 April 2018 7,212 10 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View