I´m doing methylation assays on ddPCR and have to design my assay on bisulfite converted DNA by myself. I already used the Beacon Designer, but after performing our assays, we were not that happy with our results. Now we want to try another software. My reseach on the internet only gave results for software, which only design primers but no probes. Is there anyone who has experience with the design of primers and probes on bisulfite converted DNA and can suggest a software, or give some hints for a good assay design on bisulfite converted DNA concerning methylation analysis? Has anyone experience with ABI Primer Express by ThermoFisher? Does it work on bisulfite converted DNA?

Similar questions and discussions