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Questions related from Michail Yekelchyk
Hi all, I recently found out that Illumina discontinued the DNA Nextera kit, replacing it with another one. As you might know, the enzyme from this kit (Tn5 transposase, TDE 15027865) is widely...
01 January 2019 10,183 25 View
Dear scientific community, I faced an unexpected problem regarding the detection of a protein in the murine muscle stem cells. I am interested in a GPCR, which has a relatively low mRNA...
07 July 2018 4,768 4 View
Hi everyone, P-value (or fdr), calculated by the t-test, is the common criteria of statistical significance of the measurements between conditions. It is enough to have mean, SD/SEM and N to...
07 July 2018 1,903 8 View
Hi everyone! Is there a way to methylate the DNA of the living cells (in-vitro)? For example, to demethylate the DNA we can use Decitabine. Is there a compound which initiates the opposite...
01 January 1970 7,710 6 View
Dear Researchers, I have a batch of slides (mouse muscle cryosections) from a pretty precious origin, and I try to make the best use of every slide. I made a staining with an antibody (Alexa...
01 January 1970 2,709 8 View
Dear scientists, At the moment I am analysing the RNA-seq, ATAC-seq and Methylation datasets of the same mouse-derived cell-type. Methylation was done not as bisulfite sequencing, but as an...
01 January 1970 3,522 4 View