Michail Yekelchyk

6 Questions 70 Answers 0 Followers

Questions related from Michail Yekelchyk

What is the alternative for Illumina Tn5 transposase (ATAC-seq)?

Hi all, I recently found out that Illumina discontinued the DNA Nextera kit, replacing it with another one. As you might know, the enzyme from this kit (Tn5 transposase, TDE 15027865) is widely...

01 January 2019 10,181 25 View

Transgenic reporter mouse line vs Immunostaining?

Dear scientific community, I faced an unexpected problem regarding the detection of a protein in the murine muscle stem cells. I am interested in a GPCR, which has a relatively low mRNA...

07 July 2018 4,766 4 View

Calculation of the statistical significance in case of non-discrete observations?

Hi everyone, P-value (or fdr), calculated by the t-test, is the common criteria of statistical significance of the measurements between conditions. It is enough to have mean, SD/SEM and N to...

07 July 2018 1,902 8 View

In-vitro DNA methylation compound?

Hi everyone! Is there a way to methylate the DNA of the living cells (in-vitro)? For example, to demethylate the DNA we can use Decitabine. Is there a compound which initiates the opposite...

01 January 1970 7,709 6 View

H&E staining together with Immunofluorescence?

Dear Researchers, I have a batch of slides (mouse muscle cryosections) from a pretty precious origin, and I try to make the best use of every slide. I made a staining with an antibody (Alexa...

01 January 1970 2,708 8 View

Does DNA Methylation interfere with the Tn5 accessibility and gene exon structure?

Dear scientists, At the moment I am analysing the RNA-seq, ATAC-seq and Methylation datasets of the same mouse-derived cell-type. Methylation was done not as bisulfite sequencing, but as an...

01 January 1970 3,521 4 View