Mousa Vatanmakanian

4 Questions 6 Answers 0 Followers

Questions related from Mousa Vatanmakanian

Anyone has experience with bisulfite sequencing PCR conditions?

I am optimizing the PCR conditions for Bisulfite sequencing analysis. Does it need a special PCR condition different from conventional PCR? Do I really need 25 μM of primers, as I read in a...

06 June 2018 9,490 5 View

Has anyone reprogrammed cells to iPS using Doxycycline inducible OSKM cassette?

I am doing a reprogramming process to generate iPS using Doxycycline inducible OSKM polycistronic lentiviral cassette. But just 24 hours after the first treatment all the Dox treated plates (both...

03 March 2016 6,592 3 View

How many hours is optimum time for harvesting the lentivirus contained supernatant from HEK293T cells?

Hi everybody, I have transfected HEK293T cells with lentiviral producing plasmids. If I wash the cells 12 hours after transfection and replace the supernatant with fresh medium how many hours...

03 March 2016 299 4 View

How can I find a bacterial gene sequence?

I am searching for fhaB gene sequence of some bordetella pertussis strains for my research but after searching in several databases I could not find that. Can anyone tell me how I can get my...

01 January 2015 8,032 5 View