I'm trying to quantify the population of bacillus species in the soil, can anyone suggest a selective media for bacillus species?
Bactotryptone (20 gms)+ yeast extract (2%)+ 3 gms sodium chloride and 2% agar and 1 Liter water.
You could also heat-shock your soil sample at 80C for 10 minutes to select for spore-forming bacteria (Bacillus). Then you could really use any assortment of media you wanted or have available
Thank you for the information on the media. Can i know if all Bacillus species will grow on this agar and if other bacteria other than Bacillus will grow as well? If other bacteria can grow as well, is there any way to differentiate Bacillus and non-Bacillus colonies?
David Collins is correct. If you heat shock your sample at 80C and then incubate dilutions of your sample aerobically at 30-37C, you can be certain that every single colony originated with a spore. The isolates will belong to Bacillus sensu lato, although the old Bacillus genus has been subdivided into dozens of novel genera over the past couple of decades. You can use any standard off the shelf complete medium. Nutrient agar, LB agar, TSA, and the like are all acceptable. Not every Bacillus will grow on those media, but most will.
However, if you want sample is intestinal contents, should the sample still be heat shocked to select spore forming bacteria?
For spore forming bacteria , the sample must be heated at 80 C , and dilution the sample and then incubate in appropriate medium under aerobic conditions .
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