We need a taqman "assay"
I suggest using Fusarium species.
In order to get a higher concentration of DNA with Molysis extraction kits (Molzym-Coimplete5 and UMD) we are eluting columns with 50 ul, instead of 100 ul as recommended. Can it affect DNA quality?
06 July 2017 1,687 1 View
We are obtaining poor PBMC yields from heparinized blood but high yields from blood in EDTA. The blood is processed 24-36 h after collection because samples are sent by bus or plane from 1000 km....
31 December 2013 2,011 3 View
Flow cytometry DNA quantitation in rodents to investigate gain and lost of genetic material among subspecies. Is it possible? Does anyone have a protocol?
08 September 2012 5,515 4 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View
