Can anyone suggest a protocol to purify cell free DNA fragments less than 200 bp using AmpureXP beads?

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Following is XRD of iron oxide nano material synthesised using bipyridine derivatives. What are the reasons for Broad Humps?

Even after few repetitions..I am getting similar results. Is is due to organic components?

20 January 2021 9,279 6 View

What are the advantages and limitations of SBF modeling for biological entities?

Researchers from Georgia Tech have developed SBF models to represent biological mechanism. What are advantages and disadvantages of developing and using these SBF models?

29 December 2020 5,098 2 View

How to calculate all the parameter of drug permeation and drug depostion studies in skin?

Drug permeation through mouse skin Drug deposition in mouse skin Steady-state permeation rate Flux Permeability coefficient Diffusion coefficient Lag time.

10 December 2020 3,808 1 View

What are the parameters which determine the potential window of an electrolyte?

Aqueous electrolyte have a potential window of 1.2V, while organic and ionic electrolytes have higher potential windows. But what parameter is contributing to this enhancement in the respective...

19 October 2020 2,447 3 View

How do I give glucose supplements to rabbits after Alloxan intra-peritoneal dose to prevent hypoglycemic shock?

Its study for alloxan-induced diabetes in the rabbit.

14 July 2020 2,869 1 View

A public health crisis, over the coronavirus, has landed right in the nation at the fulcrum of global commerce?

Give your opnion on said question please.

09 April 2020 1,558 4 View

In finite element study on dental implants,is it necessary to find maximum and minimum principal stress or von Mises stress anslysis is good enough?

What are the stress evaluation required for a FEA study on dental implants

09 February 2020 4,382 2 View

How i can use QuiP methodology for qualitative impact assessment ?

I want to use QuiP for impact assessment, what is the process for applying this and in which situation

16 January 2020 314 3 View

How to measure the force in spring/damper of suspension system of train experimentally?

What type of force sensors can be used?

08 January 2020 4,098 1 View

What are some positive control medicine used in diabetic wound in rabbit model?

Alloxan induced diabetes in the rabbit experiment to perform wound-healing experiments.

21 December 2019 2,724 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View